Biochemistry Lab

This lab is offered as an addition to our online Biochemistry course. Complete at-home lab experiments along with lab-based questions to meet your Biochemistry lab requirements.

What you’ll learn

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Biochemistry Lab

$69

Plus membership

1 Credit

All courses include:

eTextbooks

2 to 3-day turnaround for grading

Multiple chances to improve your grade

On-demand tutoring & writing center

Student support 7 days a week

$69

Plus membership

1 Credit

All courses include:

eTextbooks

2 to 3-day turnaround for grading

Multiple chances to improve your grade

On-demand tutoring & writing center

Student support 7 days a week

Biochemistry Lab

$69

Plus membership

1 Credit

About This Course

|
ACE Approved 2023

This lab-only course is designed as a standalone addition to the Biochemistry course. Students will complete at-home laboratory experiments, track and record results, answer lab-based questions reflected in graded lab reports, and complete lab-based assessments to meet the lab requirement.

download syllabus

What You'll Learn

Analyze experimental results to determine the presence of sugars, starch, lipids, protein, and DNA in a variety of samples.

Analyze experimental data to classify solutions as hypotonic, isotonic, or hypertonic.

Calculate aerobic respiration rates of germinated and dormant millet seeds.

Isolate DNA from split peas by physically breaking down plant tissues, lysing cell membranes with detergent, and precipitating isolated DNA in alcohol.

Illustrate the formation of a protein through transcription and translation.

Extract, determine the quantity of, and test casein proteins isolated from milk.

Analyze extracted solution from malted barley for the conversion of starch to dextrins using a starch-iodide test.

Analyze experimental data to calculate the optimal pH and temperature ranges for lactase activity.

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Lab Kit

Prefer the hard copy? Simply purchase from your favorite textbook reseller; you will still get the eTextbook for free. Custom Lab Kit from Science Interactive. Create an account at https://esciencelabs.com/user/register and use the "Have a Code?" page to search for Kit LP-5008-GB-01 which is $215 (plus shipping).

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Course Details

CHEM450L

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Biochemistry Lab

Labs are provided by eScience Labs, a leading provider of at-home lab kits and online lab instructional materials and resources. Labs will cover the following topics: biological molecules, cell membrane transport, cellular respiration and, fermentation, extraction of DNA, RNA, and protein synthesis, casein extraction, metabolism of polysaccharides and anaerobic fermentation, and enzymes, temperature, ph and specificity.

Prerequisites

There are no prerequisites to take Biochemistry Lab though we highly recommend concurrent enrollment in Biochemistry (CHEM450).

Lab Objectives
Biological Molecules
  • Define macromolecule, protein, carbohydrate, lipid, and nucleic acid.
  • Explain the metabolism of simple and complex carbohydrates.
  • Discuss the reagents used to identify biological macromolecules.
  • Perform qualitative tests for lipids, sugars, proteins, and starch.
  • Analyze experimental results to determine the presence of sugars, starch, lipids, protein, and DNA in a variety of samples.
  • Identify an unknown through its composition of macromolecules.
Cell Membrane Transport
  • Describe the structure and function of the cellular plasma membrane.
  • Define simple diffusion, osmosis, and active transport.
  • Explain water movement across a membrane in hypertonic, hypotonic, and isotonic solutions.
  • Observe osmosis in living cells using potato tissue and sucrose solutions.
  • Analyze experimental data to determine if solutions are hypotonic, isotonic, or hypertonic.
  • Examine the selective permeability of a membrane to molecules of different sizes.
Cellular Respiration and Fermentation
  • Define cellular respiration, ATP, and ADP.
  • Discuss the steps of aerobic respiration and the energy that is produced.
  • Explain fermentation and the energy that is produced.
  • Measure aerobic respiration rates of germinated and dormant millet seeds.
  • Graphically analyze experimental data.
  • Examine yeast fermentation under experimental conditions.
Extraction of DNA
  • Define deoxyribonucleic acid (DNA) and describe its role in living organisms.
  • Summarize the structure of a doublestranded DNA molecule.
  • Define the three main components of a nucleotide and describe the structure of a nucleotide.
  • List the four nitrogenous bases of DNA and describe base-pairing.
  • Isolate DNA from split peas by physically breaking down plant tissues, lysing cell membranes with detergent, and precipitating isolated DNA in alcohol.
  • Record observations of the appearance and volume of DNA extracted from peas.
DNA, RNA, and Protein Synthesis
  • Define DNA, codon, and amino acid.
  • Describe the processes of transcription and translation.
  • Discuss point and frameshift mutations.
  • Illustrate the formation of a protein through transcription and translation.
  • Model the effects of a mutation during translation.
Casein Extraction
  • Isolate, determine the quantity of, and test casein proteins extracted from milk.
  • Explain why proteins interact with metal ions in solution.
  • Describe how protein folding patterns are determined by different amino acid residues.
Metabolism of Polysaccharides and Anaerobic Fermentation
  • Define metabolism, anabolism, and catabolism.
  • Describe the stages of catabolism.
  • Explain the difference between anaerobic and aerobic conditions.
  • Discuss the significance of fermentation to the beer and wine industries.
  • Extract starch from malted barley.
  • Analyze the extracted solution for the conversion of starch to dextrins using a starch-iodide test.
  • Measure the specific gravity of a solution before and after fermentation.
  • Calculate alcohol by volume.
  • Ferment wort using yeast in a controlled environment.
Enzymes, Temperature, pH and Specificity
  • Define enzyme, active site, substrate, and activation energy.
  • Describe the relationships between concentration, pH, temperature, and enzymatic activity.
  • Discuss the enzymatic reaction between lactase and lactose.
  • Demonstrate the specificity of lactase in reactions with milk and sucrose.
  • Analyze experimental data to determine the optimal pH and temperature ranges for lactase activity.
  • Relate experimental results to conditions within the human body

Your score provides a percentage score and letter grade for each course. A passing percentage is 70% or higher.

Assignments for this course include:

  • 8 Lab Worksheets
  • 8 Lab Exams


This course does not require a textbook.


Biochemistry Lab students also take:

Biochemistry

General Physics I

General Physics I Lab


Helpful resources:

Can You Take Online Science Lab Courses for College Credit?

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